Advantages of Ostro Pass-through Sample Preparation Versus Solid Supported Liquid Extraction (SSLE)

■ ■ Ostro accommodates a range of sample volumes (50-350 μL) vs. the requirement for a fixed volume using SSLE IN T RO DU C T IO N The Ostro Pass-through 96-well plate provides a novel solution for simple pass through cleanup of plasma, serum, and whole blood samples that removes protein and phospholipids with little-to-no method development. It can be quickly implemented into routine lab workflows and produces clean extracts and excellent reproducibility resulting in consistent, robust methods with high throughput. Solid supported liquid extraction (SSLE) is analogous to traditional liquid-liquid extraction (LLE) and utilizes the same water-immiscible solvent systems for analyte extraction. Instead of shaking the two immiscible phases together, in SSLE, the aqueous sample is immobilized on an inert support (diatomaceous earth, a natural siliceous product), and the organic phase flows through the support.